| Species : |
Human/Mouse/Rat |
| Source : |
E.coli |
| Tag : |
Non |
| Protein Length : |
283-396 aa |
| Form : |
Lyophilized from a 0.2 μm filtered solution of 10mM HAc-NH4Ac, 4% D-Mannitol, pH 4.0. |
| Bio-activity : |
Determined by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 40-120 ng/mL. |
| Molecular Mass : |
13.3 KDa |
| Endotoxin : |
< 0.01 EU/µg as determined by LAL test. |
| Purity : |
Greater than 95% as determined by reducing SDS-PAGE. |
| Storage : |
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| Reconstitution : |
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
