Recombinant human/bovine/porcine TGFB1 Protein, Animal-Free

• Cat.No.: TGFB1-03H
• Product Overview: High purity optimised human/bovine/porcine TGF-β1 protein. Animal-free (AOF) and carrier protein-free.

Specification

• Species: Human
• Source: E.coli
• Description: Transforming growth factor-beta 1 (TGF-β1) is a pleiotropic cytokine part of the TGF-β superfamily. TGF-β1 regulates various cellular processes, including cell proliferation, growth, differentiation, motility, and apoptosis [1]. It plays a crucial role in the immune response, tissue repair, and the epithelial-mesenchymal transition. Transforming growth factor-beta 1 is produced by various cell types, including immune cells, fibroblasts, and epithelial cells. It is synthesized and secreted as an inactive or latent complex, associated with latency-associated proteins (LAP), and targeted to the extracellular matrix [2]. It is released from latency by TGF-β activators including plasmin, matrix metalloproteases, integrins… Once the LAP cleaved, the mature transforming growth factor-beta 1 is a homodimeric protein composed of two identical subunits linked by a disulfide bond. Its amino acid sequence is composed of 390 amino acids. TGF-β1 signals through complexes of cell surface receptors including TGF-βRII/TGF-βRI and ALK-5/ALK-1. This triggers downstream signaling cascades, such as the Smad-dependent and Smad-independent pathways.
• Form: Lyophilized from acetonitrile, TFA
• Molecular Mass: 24 kDa (dimer)
• Purity: > 98%, by SDS-PAGE quantitative densitometry
• Applications: iPSC and ESC maintenance and expansion; Chemically defined media
• Reconstitution: Resuspend in 10mM HCl at > 100 μg/mL (provided with protein and free of charge), prepare single use aliquots, add carrier protein if desired and store frozen at -20 or -80 centigrade

Gene Information

• Gene Name: TGFB1 transforming growth factor beta 1 [ Homo sapiens (human) ]
• Official Symbol: TGFB1
• Synonyms: TGFB1; transforming growth factor beta 1; CED; LAP; DPD1; TGFB; IBDIMDE; TGFbeta; TGF-beta1; transforming growth factor beta-1 proprotein; TGF-beta-1; latency-associated peptide; prepro-transforming growth factor beta-1; transforming growth factor beta1
• Gene ID: 7040
• mRNA Refseq: NM_000660
• Protein Refseq: NP_000651
• MIM: 190180
• UniProt ID: P01137