Recombinant Human BRAF Protein, GST-tagged

• Cat.No.: BRAF-320H
• Product Overview: Human BRAF (NP_004324.2, 433 a.a. - 726 a.a.) partial recombinant protein with GST tag expressed in Baculovirus infected Sf21 cells.

Specification

• Description: This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene.
• Source: Sf21 cells
• Species: Human
• Tag: GST
• Form: In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
• Molecular Mass: 60 kDa
• Purity: 69 % by SDS-PAGE/CBB staining.
• Quality Control Test: Loading 1 ug protein in SDS-PAGE
• Notes: Result of activity analysis
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)

Gene Information

• Gene Name: BRAF v-raf murine sarcoma viral oncogene homolog B1 [ Homo sapiens ]
• Official Symbol: BRAF
• Synonyms: BRAF; v-raf murine sarcoma viral oncogene homolog B1; serine/threonine-protein kinase B-raf; BRAF1; p94; 94 kDa B-raf protein; proto-oncogene B-Raf; murine sarcoma viral (v-raf) oncogene homolog B1; B-Raf proto-oncogene serine/threonine-protein kinase (p94); NS7; RAFB1; B-RAF1; FLJ95109; MGC126806; MGC138284
• Gene ID: 673
• mRNA Refseq: NM_004333
• Protein Refseq: NP_004324
• MIM: 164757
• UniProt ID: P15056