Recombinant Human BTN3A1 protein, Fc-tagged

• Cat.No.: BTN3A1-736H
• Product Overview: Recombinant Human BTN3A1 (Gln30-Gly254) protein was fused to human IgG1 Fc tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Description: Butyrophilin subfamily 3 member A1 (BTN3A1) is also known as CD277 and BTF5, which belongs to the immunoglobulin superfamily and contains one B30.2/SPRY domain and two Ig-like V-type (immunoglobulin-like) domains. BTN3A1 plays a role in T-cell activation and in the adaptive immune response. Also, BTN3A1 regulates the proliferation of activated T-cells and the release of cytokines and IFNG by activated T-cells. Furthermore, BTN3A1 mediates the response of T-cells toward infected and transformed cells that are characterized by high levels of phosphorylated metabolites, such as isopentenyl pyrophosphate.
• Source: HEK293
• Species: Human
• Tag: human IgG1 Fc
• Predicted N Terminal: Gln30
• Form: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 50.8 kDa. As a result of glycosylation, the protein migrates as 58-65 kDa under reducing (R) condition, and 120 kDa under non-reducing (NR) condition (SDS-PAGE).
• Protein length: Gln30-Gly254
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: BTN3A1
• Official Symbol: BTN3A1
• Synonyms: BTN3A1; butyrophilin, subfamily 3, member A1; butyrophilin subfamily 3 member A1; BT3.1; BTF5; CD277; dJ45P21.3 (butyrophilin, subfamily 3, member A1); MGC141880
• Gene ID: 11119
• mRNA Refseq: NM_001145008
• Protein Refseq: NP_001138480
• MIM: 613593
• UniProt ID: O00481