| Species : |
Human |
| Source : |
E.coli |
| Tag : |
His |
| Form : |
Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 0.03M Acetate buffer, pH 4.0. |
| Research topic : |
Energy metabolism and body weight regulation |
| Reconstitution : |
Add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/mL. In higher concentrations the solubility of this antigen is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture. |
| Applications : |
Western blotting |
| Quality Control Test : |
BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein |
| Sequences of amino acids : |
MKHHHHHHAS DPLLGAFARR DFRKGSPQLV CSLPGPQGPP GPPGAPGPSG MMGRMGFPGK DGQDGHDGDR GDSGEEGPPG RTGNRGKPGP KGKAGAIGRA GPRGPKGVNG TPGKHGTPGK KGPKGKKGEP GLPGPCSCGS GHTKSAFSVA VTKSYPRERL PIKFDKILMN EGGHYNASSG KFVCGVPGIY YFTYDITLAN KHLAIGLVHN GQYRIRTFDA NTGNHDVASG STILALKQGD EVWLQIFYSE QNGLFYDPYW TDSLFTGFLI YADQDDPNEV |
| Storage : |
Store lyophilized protein at -20°C. Lyophilized protein remains stable until the expiry date when stored at -20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C. |
