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Recombinant Human CA2 protein, His-tagged

Cat.No. : CA2-354H
Product Overview : Recombinant Human CA2 protein (Ser2-Lys260), fused to His tag at C-terminus, was expressed in human 293 cells (HEK293).
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Description : Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes. CAs form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa), a reversible reaction that occurs rather slowly in the absence of a catalyst. One of the functions of the enzyme in animals is to interconvert carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues. The active site of most carbonic anhydrases contains a zinc ion. They are, therefore, classified as metalloenzymes. There are at least five distinct CA families (α, β, γ, δ and ε). These families have no significant amino acid sequence similarity and in most cases are thought to be an example of convergent evolution. The α-CAs are found in humans.
Carbonic anhydrase II (CA2) is also known as Carbonate dehydratase II, Carbonic anhydrase C, is one of fourteen forms of human α carbonic anhydrases. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Renal carbonic anhydrase allows the reabsorption of sodium ions in the proximal tubule. Carbonic anhydrase II has been shown to interact with Band 3 and Sodium-hydrogen antiporter 1.
Source : HEK293
Species : Human
Tag : His
Form : Lyophilized from 0.22 um filtered solution in 20 mM Tris, pH 8.0 with 150 mM NaCl, 1 mM DTT, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 30.0 kDa. The protein migrates as 30 kDa under reducing (R) condition (SDS-PAGE).
Protein length : 259
Endotoxin : Less than 1.0 EU per ug by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 centigrade to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
Gene Name : CA2
Official Symbol : CA2
Synonyms : CA2; carbonic anhydrase II; carbonic anhydrase 2; CA II; CAII; Car2; CAC; carbonic anhydrase B; carbonic anhydrase C; carbonic dehydratase; carbonate dehydratase II; CA-II
Gene ID : 760
mRNA Refseq : NM_000067
Protein Refseq : NP_000058
MIM : 611492
UniProt ID : P00918

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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Is CA2 involved in any particular pathological conditions? 07/05/2022

CA2 deficiency is a rare autosomal recessive disorder with metabolic acidosis and osteopetrosis.

What are the known inhibitors of CA2 and their clinical implications? 02/23/2022

Acetazolamide is a known inhibitor, used clinically for glaucoma and altitude sickness.

How do mutations in the CA2 gene influence enzyme activity or stability? 06/13/2021

Some mutations can lead to CA2 deficiency, affecting acid-base balance and leading to metabolic acidosis.

How does CA2's enzymatic activity compare to other carbonic anhydrase isoforms? 07/28/2019

CA2 is one of the most efficient and fastest enzymes, displaying higher activity than many other isoforms.

What role does CA2 play in the physiological regulation of pH? 07/23/2019

CA2 accelerates the reversible conversion of CO2 and water to bicarbonate and protons, aiding in pH regulation.

Are there specific cellular pathways where CA2 plays a significant role? 11/14/2018

CA2 plays a crucial role in CO2 transport and acid-base balance in various tissues.

How is CA2 expression distributed across different human tissues? 07/04/2018

CA2 is ubiquitously expressed but is particularly abundant in red blood cells and certain epithelial tissues.

Customer Reviews (3)

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05/01/2022

    Efficient sample processing, a time-saving boon for researchers.

    08/30/2021

      Professional expertise, elevates our protein research projects.

      06/08/2021

        Invaluable assistance for intricate protein experiments.

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