Recombinant Human CA4 cell lysate

• Cat.No.: CA4-2069HCL
• Product Overview: Human CA4 / Car4 / CAIV derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Human cells
• Species: Human
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
• Tag: Non

Gene Information

• Gene Name: CA4 carbonic anhydrase IV [ Homo sapiens ]
• Official Symbol: CA4
• Synonyms: CA4; carbonic anhydrase IV; retinitis pigmentosa 17 (autosomal dominant) , RP17; carbonic anhydrase 4; CAIV; Car4; CA-IV; carbonic dehydratase IV; carbonate dehydratase IV; RP17
• Gene ID: 762
• mRNA Refseq: NM_000717
• Protein Refseq: NP_000708
• MIM: 114760
• UniProt ID: P22748
• Chromosome Location: 17q23
• Pathway: Proximal tubule bicarbonate reclamation, organism-specific biosystem; Proximal tubule bicarbonate reclamation, conserved biosystem
• Function: carbonate dehydratase activity; lyase activity; metal ion binding; protein binding; zinc ion binding

Reviews

04/14/2022

Reliable partner for protein expression and analysis.

04/12/2022

Cost-effective quantitation services, a great research investment.

12/09/2020

Invaluable for our protein analysis and quantification endeavors.

Q&As

What role does CA4 play in the respiratory system?

CA4 assists in CO2 and bicarbonate regulation, pivotal for efficient respiratory gas exchange.

Which tissues or cell types predominantly express CA4?

CA4 is highly expressed in the kidney and red blood cells.

What is the primary physiological function of CA4?

CA4 catalyzes the reversible hydration of carbon dioxide, helping regulate pH and CO2 transport.

What are the known post-translational modifications on CA4?

Typical modifications like phosphorylation can be identified using techniques such as mass spectrometry.

Is CA4 implicated in any specific cellular signaling pathways?

While primarily enzymatic, any signaling roles for CA4 would require deeper investigation.

Are there known diseases or conditions associated with CA4 mutations or dysregulation?

CA4 dysregulation could relate to renal disorders or hematological abnormalities, though research is ongoing.

How does the enzymatic activity of CA4 compare to other carbonic anhydrase family members?

CA4, like other isoforms, is involved in CO2 hydration, but its specific efficiency and affinity might vary.