Recombinant Human CA4, His tagged

• Cat.No.: CA4-3839H
• Product Overview: Recombinant Human CA4 (NP_667341.1) without the pro peptide (Met 1-Lys 283), fused with a polyhistidine tag at the C-terminus, was produced in Human Cell.

Specification

• Source: Human Cells
• Species: Human
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4
• Molecular Mass: The recombinant human CA4 consists of 276 amino acids after removal of the signal peptide and has a predicted molecular mass of 31.7 kDa. In SDS-PAGE under reducing conditions, rh CA4 migrates as an approximately 30 kDa band.
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70oC.
• Storage: Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Protein length: Met1-Lys283

Gene Information

• Gene Name: CA4 carbonic anhydrase IV [ Homo sapiens ]
• Official Symbol: CA4
• Gene ID: 762
• mRNA Refseq: NM_000717
• Protein Refseq: NP_000708
• MIM: 114760
• UniProt ID: P22748

Reviews

04/14/2022

Reliable partner for protein expression and analysis.

04/12/2022

Cost-effective quantitation services, a great research investment.

12/09/2020

Invaluable for our protein analysis and quantification endeavors.

Q&As

What role does CA4 play in the respiratory system?

CA4 assists in CO2 and bicarbonate regulation, pivotal for efficient respiratory gas exchange.

Which tissues or cell types predominantly express CA4?

CA4 is highly expressed in the kidney and red blood cells.

What is the primary physiological function of CA4?

CA4 catalyzes the reversible hydration of carbon dioxide, helping regulate pH and CO2 transport.

What are the known post-translational modifications on CA4?

Typical modifications like phosphorylation can be identified using techniques such as mass spectrometry.

Is CA4 implicated in any specific cellular signaling pathways?

While primarily enzymatic, any signaling roles for CA4 would require deeper investigation.

Are there known diseases or conditions associated with CA4 mutations or dysregulation?

CA4 dysregulation could relate to renal disorders or hematological abnormalities, though research is ongoing.

How does the enzymatic activity of CA4 compare to other carbonic anhydrase family members?

CA4, like other isoforms, is involved in CO2 hydration, but its specific efficiency and affinity might vary.