| Description : |
Functions as a coactivator for aryl hydrocarbon and nuclear receptors (NR). Recruited to promoters through its contact with the N-terminal basic helix-loop-helix-Per-Arnt-Sim (PAS) domain of transcription factors or coactivators, such as NCOA2. During ER-activation acts synergistically in combination with other NCOA2-binding proteins, such as EP300, CREBBP and CARM1. Involved in the transcriptional activation of target genes in the Wnt/CTNNB1 pathway. Functions as a secondary coactivator in LEF1-mediated transcriptional activation via its interaction with CTNNB1. Coactivator function for nuclear receptors and LEF1/CTNNB1 involves differential utilization of two different activation regions. In association with CCAR1 enhances GATA1- and MED1-mediated transcriptional activation from the gamma-globin promoter during erythroid differentiation of K562 erythroleukemia cells. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
77.3 kDa |
| AA Sequence : |
MEESPLSRAPSRGGVNFLNVARTYI PNTKVECHYTLPPGTMPSASDWIGI FKVEAACVRDYHTFVWSSVPESTTD GSPIHTSVQFQASYLPKPGAQLYQF RYVNRQGQVCGQSPPFQFREPRPMD ELVTLEEADGGSDILLVVPKATVLQ NQLDESQQERNDLMQLKLQLEGQVT ELRSRVQELERALATARQEHTELME QYKGISRSHGEITEERDILSRQQGD HVARILELEDDIQTISEKVLTKEVE LDRLRDTVKALTREQEKLLGQLKEV QADKEQSEAELQVAQQENHHLNLDL KEAKSWQEEQSAQAQRLKDKVAQMK DTLGQAQQRVAELEPLKEQLRGAQE LAASSQQKATLLGEELASAAAARDR TIAELHRSRLEVAEVNGRLAELGLH LKEEKCQWSKERAGLLQSVEAEKDK ILKLSAEILRLEKAVQEERTQNQVF KTELAREKDSSLVQLSESKRELTEL RSALRVLQKEKEQLQEEKQELLEYM RKLEARLEKVADEKWNEDATTEDEE AAVGLSCPAALTDSEDESPEDMRLP PYGLCERGDPGSSPAGPREASPLVV ISQPAPISPHLSGPAEDSSSDSEAE DEKSVLMAAVQSGGEEANLLLPELG SAFYDMASGFTVGTLSETSTGGPAT PTWKECPICKERFPAESDKDALEDH MDGHFFFSTQDPFTFETRTRPLEQK LISEEDLAANDILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
