|Product Overview:||Recombinant Human CALML5(Me1~Glu146) fused with His tag at N-terminal was expressed in E. coli.|
|Description:||This gene encodes a novel calcium binding protein expressed in the epidermis and related to the calmodulin family of calcium binding proteins. Functional studies with recombinant protein demonstrate it does bind calcium and undergoes a conformational change when it does so. Abundant expression is detected only in reconstructed epidermis and is restricted to differentiating keratinocytes. In addition, it can associate with transglutaminase 3, shown to be a key enzyme in the terminal differentiation of keratinocytes.|
|Form:||100mMNaHCO3, 500mMNaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.|
|Endotoxin:||<1.0EU per 1µg (determined by the LAL method)|
|Applications:||Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
|Stability:||The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.|
|Storage:||Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.|
|Reconstitution:||Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.|
|Gene Name:||CALML5 calmodulin-like 5 [ Homo sapiens ]|
|Synonyms:||CALML5; calmodulin-like 5; calmodulin-like protein 5; calmodulin like skin protein; CLSP; calmodulin-like skin protein;|
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