Recombinant Human CAMK2G Protein, GST-Tagged

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Recombinant Human CAMK2G Protein, GST-Tagged

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Cat.No. : CAMK2G-0334H
Product Overview : Human CAMK2G (NP_751910.1, 1 a.a. - 518 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells. cells.
Description : The product of this gene is one of the four subunits of an enzyme which belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a gamma chain. Many alternatively spliced transcripts encoding different isoforms have been described but the full-length nature of all the variants has not been determined.[provided by RefSeq, Mar 2011]
Source : Insect Cell
Species : Human
Tag : GST
Form : Liquid
Bio-activity : The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate, Mg(or Mn)/ATP, and Ca/Calmodulin. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip TM3000. Substrate: GS peptide. ATP: 100 μM.
Molecular Mass : 85 kDa
Purity : 63 % by SDS-PAGE/CBB staining
Applications : Functional Study
SDS-PAGE
Storage : Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
Storage Buffer : In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
Quality Control Testing :

Loading 1 μg protein in SDS-PAGE

Result of activity analysis :

Gene Name : CAMK2G calcium/calmodulin-dependent protein kinase II gamma [ Homo sapiens ]
Official Symbol : CAMK2G
Synonyms : CAMK2G; calcium/calmodulin-dependent protein kinase II gamma; calcium/calmodulin dependent protein kinase (CaM kinase) II gamma, CAMKG; calcium/calmodulin-dependent protein kinase type II subunit gamma; caMK-II subunit gamma; calcium/calmodulin-dependent protein kinase (CaM kinase) II gamma; CAMK; CAMKG; CAMK-II; FLJ16043; MGC26678;
Gene ID : 818
mRNA Refseq : NM_001204492
Protein Refseq : NP_001191421
MIM : 602123
UniProt ID : Q13555

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