|Product Overview :
||Human CAMK2G (NP_751910.1, 1 a.a. - 518 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells. cells.
||The product of this gene is one of the four subunits of an enzyme which belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a gamma chain. Many alternatively spliced transcripts encoding different isoforms have been described but the full-length nature of all the variants has not been determined.[provided by RefSeq, Mar 2011]
||The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate, Mg(or Mn)/ATP, and Ca/Calmodulin. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip TM3000. Substrate: GS peptide. ATP: 100 μM.
|Molecular Mass :
||63 % by SDS-PAGE/CBB staining
||Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
|Storage Buffer :
||In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)