Recombinant Human CAMKK1, GST-tagged, Active

• Cat.No.: CAMKK1-269H
• Product Overview: Recombinant full-length human CAMKK1 was expressed by baculovirus inSf9insect cells using a N-terminal GST tag. MW = 94 kDa.

Specification

• Cat. No.: CAMKK1-269H
• Description: CAMKK1 or CAMKKα is a Ca(2+)/calmodulin-dependent protein kinase that activates CaM-kinases I and IV via phosphorylation of their Thr(177) and Thr(196) residues, respectively. Recent studies have shown that the activity of CAMKK1 is decreased upon phosphorylation by cAMP-dependent protein kinase (PKA) The CAMKKalpha has been identified in intact cells as AMPKKs, predicting a significant role for this kinase in regulating AMPK activity in vivo. It has been shown that 2-deoxyglucose- and ionomycin-stimulated AMPK activity is substantially reduced in HeLa cells transfected with small interfering RNAs specific for CAMKKα.
• Source: Sf9 insect cells using baculovirus.
• Sequence: Full-length.
• Applications: Kinase Assay, Western Blot.
• Storage And Stability: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Gene Information

• Gene Name: CAMKK1 calcium/calmodulin-dependent protein kinase kinase 1, alpha [ Homo sapiens ]
• Synonyms: CAMKK1; calcium/calmodulin-dependent protein kinase kinase 1, alpha; CAMKKA; MGC34095; DKFZp761M0423; calcium/calmodulin-dependent protein kinase 1 alpha; CAMKK alpha protein; EC 2.7.11.17; OTTHUMP00000115904; Calcium/calmodulin-dependent protein kinase kinase alpha; CaM-KK alpha; CaM-kinase IV kinase; CaM-kinase kinase alpha; CaMKK alpha
• Gene ID: 84254
• mRNA Refseq: NM_032294
• Protein Refseq: NP_115670
• MIM: 611411
• UniProt ID: Q8N5S9
• Chromosome Location: 17p13.2
• Pathway: Adipocytokine signaling pathway
• Function: ATP binding; calmodulin binding; calmodulin-dependent protein kinase activity; nucleotide binding; IEA; transferase activity