Recombinant Human CARD8 Protein, GST-Tagged

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Recombinant Human CARD8 Protein, GST-Tagged

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Cat.No. : CARD8-0396H
Product Overview : Human CARD8 full-length ORF (AAH56891.1, 1 a.a. - 392 a.a.) recombinant protein with GST-tag at N-terminal.
Description : The protein encoded by this gene belongs to the caspase recruitment domain (CARD)-containing family of proteins, which are involved in pathways leading to activation of caspases or nuclear factor kappa-B (NFKB). This protein may be a component of the inflammasome, a protein complex that plays a role in the activation of proinflammatory caspases. It is thought that this protein acts as an adaptor molecule that negatively regulates NFKB activation, CASP1-dependent IL1B secretion, and apoptosis. Polymorphisms in this gene may be associated with a susceptibility to rheumatoid arthritis. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]
Source : Wheat Germ
Species : Human
Tag : GST
Molecular Mass : 70.4 kDa
AA Sequence : MEKKECPEKS SSSEEELPRR DSGSSRNIDA SKLIRLQGSR KLLVDNSIRE LQYTKTGIFF QAEACVTNDT VYRELPCVSE TLCDISHFFQ EDDETEAEPL LFRAVPECQL SGGDIPSVSE EQESSEGQDS GDICSEENQI VSSYASKVCF EIEEDYKNRQ FLGPEGNVDV ELIDKSTNRY SVWFPTAGWY LWSATGLGFL VRDEVTVTIA FGSWSQHLAL DLQHHEQWLV GGPLFDVTAE PEEAVAEIHL PHSISLQAGE VDVSWFLVAH FKNEGMVLEH PARVEPFYAV LESPSFSLMG ILLRIASGTR LSIPITSNTL IYYHPHPEDI KFHLYLVPSD ALLTKAIDDE EDRFHGVRLQ TSPPMEPLNF GSSYIVSNSA NLKVMPKWIS SL
Applications : Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
Storage : Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
Storage Buffer : 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Quality Control Testing :

12.5% SDS-PAGE Stained with Coomassie Blue.

Gene Name : CARD8 caspase recruitment domain family, member 8 [ Homo sapiens ]
Official Symbol : CARD8
Synonyms : CARD8; caspase recruitment domain family, member 8; caspase recruitment domain-containing protein 8; CARDINAL; Dakar; KIAA0955; NDPP; TUCAN; apoptotic protein NDPP1; CARD inhibitor of NF-kappaB-activating ligands; tumor up-regulated CARD-containing antagonist of CASP9; tumor up-regulated CARD-containing antagonist of caspase nine; DACAR; DAKAR; NDPP1; FLJ18119; FLJ18121; MGC57162; DKFZp779L0366;
Gene ID : 22900
mRNA Refseq : NM_001184900
Protein Refseq : NP_001171829
MIM : 609051
UniProt ID : Q9Y2G2

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