Recombinant Human Casein Kinase 1, Alpha 1, GST-His

• Cat.No.: CSNK1A1-741H
• Product Overview: Recombinant Human CK1-alpha1 Amino acids M1-F365, N-terminally fused to GST-HIS6-Thrombin cleavage site was expressed in Sf9 cells. MW = 71,434 Da.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: 1-365 a.a.
• Description: Casein kinase 1, alpha 1 has been shown to interact with Centaurin, alpha 1 and AXIN1.
• Purification: One-step affinity purification using GSH-agarose.
• Product Identity: CK1-alpha1 was confirmed as human CK1-alpha1 by mass spectroscopy LC-ESI-MS/MS (Protagen AG, Germany).
• Storage Buffer: 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol.
• Concentration: 0.058 μg/μl (Bradford method using BSA [Sigma, cat# A-7638, Lot 79H7641] as standard protein).
• Specific Activity: 3 pmol/μg×min.
• Storage: -80°C. Avoid repeated freeze-thaw cycles!

Gene Information

• Gene Name: CSNK1A1 casein kinase 1, alpha 1 [ Homo sapiens ]
• Synonyms: CSNK1A1; casein kinase 1, alpha 1; CK1; HLCDGP1; PRO2975; casein kinase I isoform alpha; Names; casein kinase I isoform alpha; CKI-alpha; OTTHUMP00000224000; down-regulated in lung cancer; EC 2.7.11.1
• Gene ID: 1452
• mRNA Refseq: NM_001025105
• Protein Refseq: NP_001020276
• MIM: 600505
• UniProt ID: P48729
• Chromosome Location: 5q32
• Pathway: Hedgehog signaling pathway; Wnt signaling pathway
• Function: ATP binding; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; transferase activity