Active Recombinant Human CASP14 Protein, His tagged

• Cat.No.: CASP14-470H
• Product Overview: Produced in E. coli. Human caspase-14 (2-242 aa) fused to a polyhistidine tag at the N-terminus.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 2-242 aa
• Description: This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This caspase has been shown to be processed and activated by caspase 8 and caspase 10 in vitro, and by anti-Fas agonist antibody or TNF-related apoptosis inducing ligand in vivo. The expression and processing of this caspase may be involved in keratinocyte terminal differentiation, which is important for the formation of the skin barrier.
• Form: Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5 % mannitol.
• Applications: Useful tool to study the enzyme regulation and kinetics, cleave target substrates, screen for inhibitors.
• Storage: Long Term Storage: -80 centigrade Centrifuge vial before opening. For prolonged storage, dilute to working aliquots and store at -80 centigrade. Avoid freeze/thaw cycles.
• Shipping: Blue Ice
• Reconstitution: Reconstitute in sterile water.

Gene Information

• Gene Name: CASP14 caspase 14, apoptosis-related cysteine peptidase [ Homo sapiens (human) ]
• Official Symbol: CASP14
• Synonyms: CASP14; caspase 14, apoptosis-related cysteine peptidase; caspase 14, apoptosis related cysteine protease; caspase-14; apoptosis related cysteine protease; MGC119078; MGC119079; MICE; CASP-14; apoptosis-related cysteine protease; caspase 14, apoptosis-related cysteine protease
• Gene ID: 23581
• mRNA Refseq: NM_012114
• Protein Refseq: NP_036246
• MIM: 605848
• UniProt ID: P31944