Recombinant Human Cathepsin L2, His-tagged

• Cat.No.: CTSL2-751H
• Product Overview: Recombinant human procathepsin V (amino acids 18-334) was fused at the N-terminus to a CD33 signal sequence (amino acids 1-16) and at the C-terminus to a His•Tag sequence and expressed inNSOcells. MW =38.7 kDa.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: 18-334 a.a.
• Description: Cathepsin L2, also known as CTSL2, Cathepsin V, CATL2, and CTSV, is a member of the peptidase C1 family. It is predominantly expressed in the thymus and testis. Cathepsin V is also expressed in corneal epithelium, and to a lesser extent in conjuctival epithelium and skin. Cathepsin V is a lysosomal cysteine proteinase that may play an important role in corneal physiology. It has about 75% protein sequence identity to murine cathepsin L. The fold of this enzyme is similar to the fold adopted by other members of the papain superfamily of cysteine proteases. Cathepsins K and S are papain-like cysteine proteases with known elastolytic activities, and both enzymes have been identified in macrophages present in plaque areas of diseased blood vessels. Cathepsin V is the third elastolytic cysteine protease which exhibits the most potent elastase activity yet described among human proteases and that Cathepsin V is present in atherosclerotic plaque specimens. Increase of cathepsins V and K activities may accelerate the destruction of the elastin matrix in diseased arteries.
• Purity: ≥95% by SDS-PAGE.
• Form: Frozen liquid. In 800 mM NaCl, 25 mM sodium acetate, 0.8 M NaCl, pH 5.0. Please refer to vial label for lot-specific concentration.
• Endotoxins: ≤1.0 EU/µg Cathepsin V.
• Specific Activity: ≥300 units/µg.
• Unit Definition: One unit is defined as the amount of enzyme that will cleave 1 pmol the fluorescent substrate Z-LR-AMC per min at 25°C, pH 5.5.
• Toxicity: Standard Handling (MSDS available upon request).
• Storage: ≤-70℃. Avoid freeze/thaw. To activate rhCathepsin V, pre-incubate it at 20 µg/ml in 25 mM NaOAc, 100 mM NaCl, 5 mM DTT, pH 5.5 at room temperature. Following initial thaw aliquot and freeze (-70℃).

Gene Information

• Gene Name: CTSL2 cathepsin L2 [ Homo sapiens ]
• Synonyms: CTSL2; cathepsin L2; CTSU; CTSV; CATL2; MGC125957; EC 3.4.22.43; Cathepsin L2; Cathepsin V; Cathepsin U; UNQ268/PRO305
• Gene ID: 1515
• mRNA Refseq: NM_001333
• Protein Refseq: NP_001324
• MIM: 603308
• UniProt ID: O60911
• Chromosome Location: 9q22.2
• Pathway: Lysosome
• Function: cysteine-type endopeptidase activity; peptidase activity; protein binding