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Recombinant Human CBLB Protein, His-Avi-tagged, Biotinylated

Cat.No.: CBLB-19H
Product Overview: Biotinylated Human CBLB, His Tag is expressed from E.coli. It contains AA Ala 39 - Phe 426 (Accession # Q13191-1). This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag.
Source: E.coli
Species: Human
Tag : His-Avi
Predicted N Terminal: His
Form: Lyophilized from 0.22 um filtered solution in 40 mM HEPES, 120 mM NaCl, pH7.5, 10% trehalose.
Molecular Mass: The protein has a calculated MW of 47.8 kDa. The protein migrates as 45 kDa and 46 kDa under reducing condition (SDS-PAGE) due to glycosylation.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >90% as determined by reduced SDS-PAGE.
Characteristic: Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.6-1 as determined by the HABA assay.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: CBLB Cbl proto-oncogene, E3 ubiquitin protein ligase B [ Homo sapiens ]
Official Symbol: CBLB
Synonyms: CBLB; Cbl proto-oncogene, E3 ubiquitin protein ligase B; Cas Br M (murine) ecotropic retroviral transforming sequence b , Cas Br M (murine) ectropic retroviral transforming sequence b; E3 ubiquitin-protein ligase CBL-B; Cbl b; RNF56; RING finger protein 56; SH3-binding protein CBL-B; signal transduction protein CBL-B; casitas B-lineage lymphoma proto-oncogene b; Cas-Br-M (murine) ectropic retroviral transforming sequence b; Cas-Br-M (murine) ecotropic retroviral transforming sequence b; Cbl-b; Nbla00127; FLJ36865; FLJ41152; DKFZp779A0729; DKFZp779F1443; DKFZp686J10223;
Gene ID: 868
mRNA Refseq: NM_170662
Protein Refseq: NP_733762
MIM: 604491
UniProt ID: Q13191

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