Recombinant Human Cbp/P300-Interacting Transactivator, With Glu/Asp-Rich Carboxy-Terminal Domain, 2, His-tagged

• Cat.No.: CITED2-6919H
• Product Overview: Recombinant CITED2 protein, fused to His-tagged, was expressed in E. coli and purified by using conventional chromatography techniques. MW: 29.5 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-270aa
• Description: CITED2 is an important transcriptional cofactor involved in multiple organ development. It is a cAMP-responsive element-binding protein (CBP)/p300 interacting transcriptional modulator and a proposed negative regulator for hypoxia-inducible factor (HIF)-1a through its competitive binding with HIF-1a to CBP/p300. CITED2 is necessary for mouse fetal liver hematopoiesis and is required for the proper formation of the hyaloid vasculature and for lens morphogenesis.
• Form: Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 0.1M NaCl, 50% glycerol, 1mM DTT.
• Molecular Mass: 29.5 kDa (278 aa), confirmed by MALDI-TOF.
• AA Sequence: MADHMMAMNH GRFPDGTNGL HHHPAHRMGM GQFPSPHHHQ QQQPQHAFNA LMGEHIHYGA GNMNATSGIR HAMGPGTVNG GHPPSALAPA ARFNNSQFMG PPVASQGGSL PASMQLQKLN NQYFNHHPYP HNHYMPDLHP AAGHQMNGTN QHFRDCNPKH SGGSSTPGGS GGSSTPGGSG SSSGGGAGSS NSGGGSGSGN MPASVAHVPA AMLPPNVIDT DFIDEEVLMS LVIEMGLDRI KELPELWLGQ NEFDFMTDFV CKQQPSRVSC LEHHHHHH
• Endotoxin: < 1.0 eu per 1 μg of protein (determined by lal
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: CITED2 Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 2 [ Homo sapiens ]
• Official Symbol: CITED2
• Synonyms: CITED2; Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 2; cbp/p300-interacting transactivator 2; MRG1; MRG-1; MSG1-related gene 1; MSG-related protein 1; melanocyte-specific gene 1-related gene 1; ASD8; VSD2; P35SRJ
• Gene ID: 10370
• mRNA Refseq: NM_001168388
• Protein Refseq: NP_001161860
• MIM: 602937
• UniProt ID: Q99967
• Chromosome Location: 6q23.3
• Pathway: HIF-1-alpha transcription factor network, organism-specific biosystem; HIF-2-alpha transcription factor network, organism-specific biosystem
• Function: LBD domain binding; RNA polymerase II activating transcription factor binding; SMAD binding; chromatin binding; protein binding; sequence-specific DNA binding transcription factor activity; transcription coactivator activity; transcription corepressor activity; transcription corepressor activity