Recombinant Human CBS, His-tagged

• Cat.No.: CBS-10765H
• Product Overview: Recombinant Human CBS protein, fused to His-tag, was expressed in E.coli and purified by Ni-sepharose.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 206-551a.a.
• Description: The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5 UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene.
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 300mM Imidazole and 0.7% Sarcosyl, 15%glycerol.

Gene Information

• Gene Name: CBS cystathionine-beta-synthase [ Homo sapiens ]
• Official Symbol: CBS
• Synonyms: CBS; cystathionine-beta-synthase; cystathionine beta-synthase; HIP4; beta-thionase; serine sulfhydrase; methylcysteine synthase
• Gene ID: 875
• mRNA Refseq: NM_000071
• Protein Refseq: NP_000062
• MIM: 613381
• UniProt ID: P35520
• Chromosome Location: 21q22.3
• Pathway: Cysteine and methionine metabolism, organism-specific biosystem; Cysteine and methionine metabolism, conserved biosystem; Cysteine biosynthesis, homocysteine + serine => cysteine, organism-specific biosystem; Cysteine biosynthesis, homocysteine + serine => cysteine, conserved biosystem; Cysteine formation from homocysteine, organism-specific biosystem
• Function: cystathionine beta-synthase activity; cystathionine beta-synthase activity; enzyme binding; heme binding; heme binding; lyase activity; metal ion binding; modified amino acid binding; protein binding; protein homodimerization activity; pyridoxal phosphate