Recombinant Human CCL11 protein
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Cat.No. : | CCL11-3564H Optional Service: Optional requirements on this protein |
Product Overview : | Recombinant Human CCL11 protein was expressed in E.coli. |
Description : | Human CCL11 is belonging to the CC chemokine family. It is encoded by the gene CCL11. CCL11 was first purified from bronchoalveolar lavage fluid of guinea pigs. It was a strong and specific eosinophil chemoattractant in vitro. It can directly chemotactic for eosinophils, but not for monocytes or neutrophils. Human CCL11 is approximately 63 % identical at the amino acid level to murine CCL11. In addition, CCL11 also shows about 60 % amino acid sequence identity to human MCPs. CCR3 has been identified to be a specific CCL11 receptor. |
Source : | E.coli |
Species : | Human |
Form : | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl. |
Bio-activity : | Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood eosinophils is in a concentration range of 0.1-10.0 ng/ml. |
Molecular Mass : | Approximately 8.4 kDa, a single non-glycosylated polypeptide chain containing 74 amino acids. |
Protein length : | 74 |
AA Sequence : | GPASVPTTCC FNLANRKIPL QRLESYRRIT SGKCPQKAVI FKTKLAKDIC ADPKKKWVQD SMKYLDQKSP TPKP |
Endotoxin : | Less than 1 EU/μg of rHuEotaxin/CCL11 as determined by LAL method. |
Purity : | >97% by SDS-PAGE and HPLC analyses. |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
Reconstitution : | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
SDS-PAGE of CCL11-3564H : |
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Gene Name : | CCL11 |
Official Symbol : | CCL11 |
Synonyms : | CCL11; chemokine (C-C motif) ligand 11; SCYA11, small inducible cytokine subfamily A (Cys Cys), member 11 (eotaxin); eotaxin; eotaxin 1; MGC22554; eotaxin-1; C-C motif chemokine 11; small-inducible cytokine A11; eosinophil chemotactic protein; small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin); SCYA11; |
Gene ID : | 6356 |
mRNA Refseq : | NM_002986 |
Protein Refseq : | NP_002977 |
MIM : | 601156 |
UniProt ID : | P51671 |
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◆ Lysates
CCL11-7735HCL | Recombinant Human CCL11 293 Cell Lysate | +Inquiry |
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CCL1CCL11Ccl12CCL13CCL14CCL15CCL16CCL17CCL18CCL19CCL2CCL20CCL21Ccl21cCCL22CCL23CCL24CCL25CCL26CCL27Ccl27aCCL28CCL3CCL3L1Ccl4CCL5Ccl6CCL7CCL8Ccl9CCL4L2Ccl21aCCL4L1CCL3L3Ccl21bCcl27b
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