Recombinant Human CCL11 protein CCL11-3564H

Recombinant Human CCL11 protein

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Cat.No. : CCL11-3564H Optional Service: Optional requirements on this protein
Product Overview : Recombinant Human CCL11 protein was expressed in E.coli.
Description : Human CCL11 is belonging to the CC chemokine family. It is encoded by the gene CCL11. CCL11 was first purified from bronchoalveolar lavage fluid of guinea pigs. It was a strong and specific eosinophil chemoattractant in vitro. It can directly chemotactic for eosinophils, but not for monocytes or neutrophils. Human CCL11 is approximately 63 % identical at the amino acid level to murine CCL11. In addition, CCL11 also shows about 60 % amino acid sequence identity to human MCPs. CCR3 has been identified to be a specific CCL11 receptor.
Source : E.coli
Species : Human
Form : Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Bio-activity : Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood eosinophils is in a concentration range of 0.1-10.0 ng/ml.
Molecular Mass : Approximately 8.4 kDa, a single non-glycosylated polypeptide chain containing 74 amino acids.
Protein length : 74
AA Sequence : GPASVPTTCC FNLANRKIPL QRLESYRRIT SGKCPQKAVI FKTKLAKDIC ADPKKKWVQD SMKYLDQKSP TPKP
Endotoxin : Less than 1 EU/μg of rHuEotaxin/CCL11 as determined by LAL method.
Purity : >97% by SDS-PAGE and HPLC analyses.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 centigrade. Further dilutions should be made in appropriate buffered solutions.
SDS-PAGE of CCL11-3564H :

Gene Name : CCL11
Official Symbol : CCL11
Synonyms : CCL11; chemokine (C-C motif) ligand 11; SCYA11, small inducible cytokine subfamily A (Cys Cys), member 11 (eotaxin); eotaxin; eotaxin 1; MGC22554; eotaxin-1; C-C motif chemokine 11; small-inducible cytokine A11; eosinophil chemotactic protein; small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin); SCYA11;
Gene ID : 6356
mRNA Refseq : NM_002986
Protein Refseq : NP_002977
MIM : 601156
UniProt ID : P51671
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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