| Cat.No. : |
CCL3L1-68H
Optional Service: Optional requirements on this protein
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| Product Overview : |
Recombinant Human chemokine (C-C motif) ligand 3-like 1 was expressed inE. Coli. |
| Description : |
Recombinant Human LD78beta is a 7.8 kda protein containing 70 amino acid residues, which is closely related to MIP-1alpha. LD78b has enhanced receptor binding affinities to CCR5 and the beta-chemokine receptorD6 and has been shown to exhibit activities in HIV supression assays. |
| Source : |
E. Coli. |
| Amino Acid Sequence : |
APLAADTPTA CCFSYTSRQI PQNFIADYFETSSQCSKPSV IFLTKRGRQV CAD PSEEWVQKYVSDLELSA. |
| Supplied : |
Lyophilized powder with no additives. |
| Purity : |
>98% by SDS-Page analysis. Endotoxin level is less than 0.1ng per ug (1EU/ug) of protein. |
| Reactivity : |
Determined by its ability to chemoattract human monocytes using a concentration range of 1.0-10.0 ng/ml. |
| Note : |
If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA or HSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >20ug/ml. |
| Reconstitution : |
LyophilizedLD78-beta should be reconstituted in water to a concentration of 0.1-1mg/ml. We recommend a quick spin or genlte tap on table top followed by reconstitution in water. Allow to set at least 30 minutes at 4 °C, mix well. If desired, tighten cap and centrifuge for 1 minute at 1000rpm to concentrate material in vial. This solution can be diluted into other buffered solutions or stored at 4 °C for up to 1 week or at -20 °C for future use. |
| Storage : |
The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 °C. Reconstituted LD78B should be stored in working aliquots at -20 °C. avoid frequent freeze thaw cycles. |
| PDB rendering based on 1b50. : |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.