Recombinant Human CCND1 Protein (Lue65-Arg245), His tagged

• Cat.No.: CCND1-1140H
• Product Overview: Recombinant human CCND1 (Lue65-Arg245) fused with His tag was expressed in E. coli.

Specification

• Description: Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Lyophilized powder/frozen liquid
• Molecular Mass: 21.92 kDa
• Protein Length: Lue65-Arg245
• Purity: >90% as determined by SDS-PAGE.
• Notes: For research use only.
• Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles.
  Store at 2 to 8 centigrade for one week.
  Store at -20 to -80 centigrade for twelve months from the date of receipt.
• Storage Buffer: Supplied as solution form in PBS pH 7.5 or lyophilized from PBS pH 7.5.
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Shipping: They are shipped out with dry ice/blue ice unless customers require otherwise.

Gene Information

• Gene Name: CCND1 cyclin D1 (PRAD1: parathyroid adenomatosis 1) [ Homo sapiens (human) ]
• Official Symbol: CCND1
• Synonyms: CCND1; cyclin D1 (PRAD1: parathyroid adenomatosis 1); BCL1, cyclin D1 (PRAD1: parathyroid adenomatosis 1), D11S287E, PRAD1; B cell CLL/lymphoma 1; G1/S specific cyclin D1; parathyroid adenomatosis 1; U21B31; BCL-1; PRAD1; D11S287E
• Gene ID: 893
• mRNA Refseq: NM_053056
• Protein Refseq: NP_444284
• MIM: 168461
• UniProt ID: P24385