Recombinant Human CCNE1 Protein, GST-Tagged

• Cat.No.: CCNE1-0665H
• Product Overview: Human CCNE1 full-length ORF (AAH35498.1, 1 a.a. - 410 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. [provided by RefSeq, Apr 2016]
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 70.84 kDa
• AA Sequence: MPRERRERDAKERDTMKEDGGAEFS ARSRKRKANVTVFLQDPDEETAKID RTARDQCGSQPWDNNAVCADPCSLI PTPDKEDDDRVYPNSTCKPRIIAPS RGSPLPVLSWANREEVWKIMLNKEK TYLRDQHFLEQHPLLQPKMRAILLD WLMEVCEVYKLHRETFYLAQDFFDR YMATQENVVKTLLQLIGISSLFIAA KLEEIYPPKLHQFAYVTDGACSGDE ILTMELMIMKALKWRLSPLTIVSWL NVYMQVAYLNDLHEVLLPQYPQQIF IQIAELLDLCVLDVDCLEFPYGILA ASALYHFSSSELMQKVSGYQWCDIE NCVKWMVPFAMVIRETGSSKLKHFR GVADEDAHNIQTHRDSLDLLDKARA KKAMLSEQNRASPLPSGLLTPPQSG KKQSSGPEMA
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: CCNE1 cyclin E1 [ Homo sapiens ]
• Official Symbol: CCNE1
• Synonyms: CCNE1; cyclin E1; CCNE; G1/S-specific cyclin-E1; cyclin Es; cyclin Et
• Gene ID: 898
• mRNA Refseq: NM_001238
• Protein Refseq: NP_001229
• MIM: 123837
• UniProt ID: P24864