Recombinant Human CCNT2 293 Cell Lysate

• Cat.No.: CCNT2-7701HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for cyclin T2 (CCNT2), transcript variant a is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: CCNT2 cyclin T2 [ Homo sapiens ]
• Official Symbol: CCNT2
• Synonyms: CCNT2; cyclin T2; cyclin-T2; cyclin T2a; cyclin T2b; SDS-stable vimentin-bound DNA fragment HEF42VIM22; subunit of positive elongation transcription factor b; CYCT2; FLJ13583; FLJ90560; MGC134840
• Gene ID: 905
• mRNA Refseq: NM_001241
• Protein Refseq: NP_001232
• MIM: 603862
• UniProt ID: O60583
• Chromosome Location: 2q21.3
• Pathway: Disease, organism-specific biosystem; Formation of HIV-1 elongation complex in the absence of HIV-1 Tat, organism-specific biosystem; Formation of RNA Pol II elongation complex, organism-specific biosystem; Gene Expression, organism-specific biosystem; HIV Infection, organism-specific biosystem; HIV Life Cycle, organism-specific biosystem; HIV-1 elongation arrest and recovery, organism-specific biosystem
• Function: protein kinase binding