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Active Recombinant Human CD14 protein, His-tagged

Cat.No. : CD14-2109H
Product Overview : Recombinant Human CD14 (Met 1-Met 344) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).
  • Specification
  • Gene Information
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Source : HEK293
Species : Human
Tag : His
Form : Lyophilized from sterile 50 mM Tris, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Bio-activity : Measured by its ability to enhance LPS­stimulated IL­8 secretion by THP­1 human acute monocytic leukemia cells. The ED50 for this effect is typically 25-95 ng/mL.
Molecular Mass : The secreted recombinant human CD14 consists of 336 amino acids and has a predicted molecular mass of 36.5 kDa. The apparent molecular mass of rhCD14 is approximately 45 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Protein Length : Met 1-Met 344
Endotoxin : < 1.0 EU per μg protein as determined by the LAL method.
Purity : > 97 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
Gene Name : CD14 CD14 molecule [ Homo sapiens ]
Official Symbol : CD14
Synonyms : CD14; CD14 molecule; CD14 antigen; monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein;
Gene ID : 929
mRNA Refseq : NM_000591
Protein Refseq : NP_000582
MIM : 158120
UniProt ID : P08571

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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