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Recombinant Human CD19 protein, Fc-tagged

Cat.No. : CD19-3307H
Product Overview : Recombinant Human CD19(Pro 20 - Pro 278) fused with with a human IgG1 with a human IgG1 Fc tag tag at C-terminal was expressed in HEK293.
Source : HEK293
Species : Human
Tag : Fc
Predicted N Terminal : Pro 20
Form : Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Bio-activity : Measured by its binding ability in a functional ELISA. Immobilized Mouse anti-human CD19 Mab FMC63 or HIB19 10 μg/mL ( 100 μl/well ) can bind Human CD19 (20-278), Fc Tag with a linear ranger of 10-1000 ng/mL.Measured by its binding ability in a functional ELISA. Immobilized Human CD9, His Tag at 2 μg/mL (100 μl/well) can bind Human CD19 (20-278), Fc Tag with a linear range of 0.05-0.5 μg/mL.
Molecular Mass : Has a calculated MW of 55.1 kDa. DTT-reduced Protein migrates as 62-70 kDa in SDS-PAGE due to glycosylation.
Protein length : Pro 20 - Pro 278
Endotoxin : Less than 1.0 EU per μg of the Human CD19 (20-278), Fc Tag by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).
Gene Name : CD19 CD19 molecule [ Homo sapiens ]
Official Symbol : CD19
Synonyms : CD19; CD19 molecule; CD19 antigen; B-lymphocyte antigen CD19; differentiation antigen CD19; T-cell surface antigen Leu-12; B-lymphocyte surface antigen B4; B4; CVID3; MGC12802;
Gene ID : 930
mRNA Refseq : NM_001178098
Protein Refseq : NP_001171569
MIM : 107265
UniProt ID : P15391
Chromosome Location : 16p11.2
Pathway : Adaptive Immune System, organism-specific biosystem; Antigen Activates B Cell Receptor Leading to Generation of Second Messengers, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; B cell receptor signaling pathway, organism-specific biosystem; B cell receptor signaling pathway, conserved biosystem; BCR signaling pathway, organism-specific biosystem; Hematopoietic cell lineage, organism-specific biosystem;
Function : receptor signaling protein activity;

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