Recombinant Human CD2 Protein (Met1-Asp209), HlgG1 Fc-tagged

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Recombinant Human CD2 Protein (Met1-Asp209), HlgG1 Fc-tagged

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Cat.No. : CD2-1077H
Product Overview : Recombinant Human CD2 Protein (NP_001758.2) (Met1-Asp209) was produced by HEK293 Cells expression system. This protein was expressed with the Fc region of human IgG1 at the C-terminus.
Description : T-cell surface antigen CD2, also known as T-cell surface antigen T11/Leu-5, and SRBC, is a single-pass type I membrane protein. It contains one Ig-like C2-type domain and one Ig-like V-type domain. CD2 is a cell adhesion molecule expressed on T cells and is recognized as a target for CD48 (rats) and CD58 (humans). CD2 has been shown to set quantitative thresholds in T cell activation both in vivo and in vitro. Further, intracellular CD2 signaling pathways and networks are being discovered by the identification of several cytosolic tail binding proteins. CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain of CD2 is implicated in the signaling function. The complex of CD2 and CD58 also plays an important role in enhancing the adhesion of T lymphocytes to target cells, and promoting hyperplasia and activation of T lymphocytes. As a cell surface glycoprotein, CD2 expressed on most human T cells and natural killer (NK) cells and plays an important role in mediating cell adhesion in both T-lymphocytes and in signal transduction.
Source : HEK293
Species : Human
Tag : HlgG1 Fc
Predicted N Terminal : Lys 25
Form : Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Molecular Mass : The recombinant human CD2 consists of 423 amino acids and predicts a molecular mass of 48 kDa.
Protein length : Met1-Asp209
Endotoxin : < 1.0 EU per μg protein as determined by the LAL method.
Purity : > 90 % as determined by SDS-PAGE.
Stability : Samples are stable for up to twelve months from date of receipt at -70 centigrade.
Storage : Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Shipping : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Gene Name : CD2 CD2 molecule [ Homo sapiens ]
Official Symbol : CD2
Synonyms : CD2; CD2 molecule; LFA-3 receptor; rosette receptor; T11; SRBC; LFA-2; FLJ46032;
Gene ID : 914
mRNA Refseq : NM_001767
Protein Refseq : NP_001758
MIM : 186990
UniProt ID : P06729

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