Active Recombinant Human CD200R1 Protein, Fc-Avi-tagged, Biotinylated

• Cat.No.: CD200R1-348H
• Product Overview: Biotinylated Human CD200 R1, Fc Tag is expressed from HEK293 cells. It contains AA Ala 27 - Leu 266 (Accession # AAQ19772.1). This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc-Avi
• Predicted N Terminal: Ala 27
• Form: Lyophilized from 0.22 um filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
• Bio-activity: Immobilized Human CD200, Fc Tag at 2 ug/mL (100 uL/well) can bind Biotinylated Human CD200 R1, Fc Tag with a linear range of 0.5-7.8 ug/mL.
• Molecular Mass: The protein has a calculated MW of 55.8 kDa. The protein migrates as 70-115 KDa under reducing condition (SDS-PAGE) due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95% as determined by reduced SDS-PAGE.
• Characteristic: Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: CD200R1 CD200 receptor 1 [ Homo sapiens ]
• Official Symbol: CD200R1
• Synonyms: CD200R1; CD200 receptor 1; MOX2 receptor , MOX2R; cell surface glycoprotein CD200 receptor 1; CD200R; HCRTR2; OX2R; MOX2 receptor; CD200 cell surface glycoprotein receptor; cell surface glycoprotein OX2 receptor 1; cell surface glycoprotein receptor CD200; MOX2R
• Gene ID: 131450
• mRNA Refseq: NM_138806
• Protein Refseq: NP_620161
• MIM: 607546
• UniProt ID: Q8TD46