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Recombinant Human CD22 cell lysate

Cat.No. : CD22-1956HCL
Product Overview : Human Siglec-2 / CD22 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : CD22 CD22 molecule [ Homo sapiens ]
Official Symbol : CD22
Synonyms : CD22; CD22 molecule; CD22 antigen; B-cell receptor CD22; sialic acid binding Ig like lectin 2; SIGLEC 2; SIGLEC2; BL-CAM; T-cell surface antigen Leu-14; B-lymphocyte cell adhesion molecule; sialic acid binding Ig-like lectin 2; sialic acid-binding Ig-like lectin 2; SIGLEC-2; FLJ22814; MGC130020;
Gene ID : 933
mRNA Refseq : NM_001185099
Protein Refseq : NP_001172028
MIM : 107266
UniProt ID : P20273
Chromosome Location : 19q13.1
Pathway : B Cell Receptor Signaling Pathway, organism-specific biosystem; B cell receptor signaling pathway, organism-specific biosystem; B cell receptor signaling pathway, conserved biosystem; BCR signaling pathway, organism-specific biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Hematopoietic cell lineage, organism-specific biosystem;
Function : protein binding; sugar binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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How does CD22-targeted immunotherapy differ from traditional chemotherapy? 02/05/2023

CD22-targeted immunotherapy is more targeted, specifically attacking cancer cells, whereas chemotherapy affects both healthy and cancerous cells.

Can CD22-targeted therapies be used in combination with other cancer treatments? 01/09/2023

Yes, CD22-targeted therapies are often used in combination with chemotherapy or other immunotherapies for enhanced efficacy.

What other diseases involve CD22 as a therapeutic target? 10/08/2020

CD22-targeted therapies are being explored for autoimmune diseases like rheumatoid arthritis and systemic lupus erythematosus.

Are there any clinical applications of CD22 protein in cancer therapy? 01/11/2019

Yes, CD22 is targeted in immunotherapy for B cell malignancies like acute lymphoblastic leukemia (ALL).

What role does CD22 play in B cell function? 05/13/2018

CD22 acts as an inhibitory receptor, regulating B cell activation and signaling.

Customer Reviews (3)

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Reviews
01/15/2021

    The CD22 protein offers numerous advantages in trials and research studies, making it an excellent choice for scientists in need of reliable and high-quality experimental materials.

    06/15/2017

      Its unique properties make CD22 an invaluable tool for investigating cellular signaling pathways, protein-protein interactions, and membrane dynamics.

      02/17/2016

        With the CD22 protein and its accompanying technical assistance, I am confident that I can achieve successful outcomes for my research endeavors.

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