Recombinant Human CD22 protein, His-Avi-tagged, Biotinylated
Cat.No. : | CD22-051H |
Product Overview : | Biotinylated Recombinant Human CD22 protein(P20273-1)(Asp20-Arg687), fused with C-terminal His and Avi tag, was expressed in HEK293. |
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Species : | Human |
Source : | HEK293 |
Tag : | His&Avi |
ProteinLength : | Asp20-Arg687 |
Tag : | C-His-Avi |
Conjugation/Label : | Biotin |
Form : | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
Bio-activity : | Immobilized Biotinylated Human Siglec-2, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-Siglec-2 Antibody, hFc Tag with the EC50 of 9.2ng/ml determined by ELISA. |
Molecular Mass : | The protein has a predicted MW of 78.1 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Tris-Bis PAGE result. |
Endotoxin : | Less than 1EU per μg by the LAL method. |
Purity : | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
Storage : | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Reconstitution : | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Gene Name | CD22 CD22 molecule [ Homo sapiens ] |
Official Symbol | CD22 |
Synonyms | CD22; CD22 molecule; CD22 antigen; B-cell receptor CD22; sialic acid binding Ig like lectin 2; SIGLEC 2; SIGLEC2; BL-CAM; T-cell surface antigen Leu-14; B-lymphocyte cell adhesion molecule; sialic acid binding Ig-like lectin 2; sialic acid-binding Ig-like lectin 2; SIGLEC-2; FLJ22814; MGC130020; |
Gene ID | 933 |
mRNA Refseq | NM_001185099 |
Protein Refseq | NP_001172028 |
MIM | 107266 |
UniProt ID | P20273 |
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Not For Human Consumption!
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