Active Recombinant Human CD226 protein, His-Avi-tagged, Low Endotoxin

• Cat.No.: CD226-578H
• Product Overview: Recombinant Human CD226 protein(Q15762)(19-247 aa), fused with C-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: 19-247 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human DNAM-1, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human CD155, mFc Tag with the EC50 of 0.45μg/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 28.9 kDa. Due to glycosylation, the protein migrates to 45-70 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: CD226 CD226 molecule [ Homo sapiens ]
• Official Symbol: CD226
• Synonyms: CD226; CD226 molecule; CD226 antigen; DNAM 1; DNAM1; PTA1; TLiSA1; adhesion glycoprotein; DNAX accessory molecule 1; DNAX accessory molecule-1; platelet and T cell activation antigen 1; T lineage-specific activation antigen 1 antigen; DNAM-1
• Gene ID: 10666
• mRNA Refseq: NM_006566
• Protein Refseq: NP_006557
• MIM: 605397
• UniProt ID: Q15762