Active Recombinant Human CD24 protein, mFc-tagged, Low Endotoxin

• Cat.No.: CD24-3061H
• Product Overview: Recombinant Human CD24 protein(P25063-1)(Ser27-Gly59), fused with C-terminal mFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: mFc
• Protein Length: 27-59 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human CD24, mFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD24 Antibody, hFc Tag with the EC50 of 54.3ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 29.5 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: CD24 CD24 molecule [ Homo sapiens ]
• Official Symbol: CD24
• Synonyms: CD24; CD24 molecule; CD24 antigen (small cell lung carcinoma cluster 4 antigen); signal transducer CD24; CD24A; FLJ22950; FLJ43543; MGC75043
• Gene ID: 100133941
• mRNA Refseq: NM_013230
• Protein Refseq: NP_037362
• MIM: 600074
• UniProt ID: P25063