Active Recombinant Human CD27 Protein, Fc & Avi-tagged, Biotinylated

• Cat.No.: CD27-57H
• Product Overview: Active Recombinant Human CD27 Protein(P26842)(Thr21-Ile192) is expressed from HEK293 with hFc (IgG1) tag and Avi tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&Fc
• Protein Length: 21-192 aa
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Human CD27 Ligand (Trimer), His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CD27, hFc Tag with the EC50 of 0.12μg/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 47.01 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 1 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: CD27 CD27 molecule [ Homo sapiens ]
• Official Symbol: CD27
• Synonyms: CD27; CD27 molecule; TNFRSF7, tumor necrosis factor receptor superfamily, member 7; CD27 antigen; S152; Tp55; CD27L receptor; T cell activation antigen S152; T-cell activation antigen CD27; tumor necrosis factor receptor superfamily, member 7; T14; TNFRSF7; MGC20393
• Gene ID: 939
• mRNA Refseq: NM_001242
• Protein Refseq: NP_001233
• MIM: 186711
• UniProt ID: P26842