Recombinant Human CD27 Protein, Fc-tagged

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Recombinant Human CD27 Protein, Fc-tagged

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Cat.No. : CD27-180H
Product Overview : Recombinant human CD27 (P26842) (Met1-Ile192) was expressed, fused with the Fc region of rabbit IgG at the C-terminus.
Source : HEK293
Species : Human
Tag : Fc
Predicted N Terminal : Ala 20
Form : Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Bio-activity : Measured by its binding ability in a functional ELISA.Immobilized human CD27-his at 10 μg/mL (100 μl/well) can bind biotinylated human CD70-Fc, The EC50 of biotinylated human CD70-Fc is 95-150 ng/mL.
Molecular Mass : The recombinant human CD27/rFc is a disulfide-linked homodimer. The reduced monomer comprises 396 amino acids and has a predicted molecular mass of 44.4 kDa. The apparent molecular mass of the protein is approximately 63 kDa in SDS-PAGE under reducing conditions.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Purity : >95 % as determined by SDS-PAGE.
Stability : Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage : Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name : CD27 CD27 molecule [ Homo sapiens ]
Official Symbol : CD27
Synonyms : CD27; CD27 molecule; TNFRSF7, tumor necrosis factor receptor superfamily, member 7; CD27 antigen; S152; Tp55; CD27L receptor; T cell activation antigen S152; T-cell activation antigen CD27; tumor necrosis factor receptor superfamily, member 7; T14; TNFRSF7; MGC20393;
Gene ID : 939
mRNA Refseq : NM_001242
Protein Refseq : NP_001233
MIM : 186711
UniProt ID : P26842
Chromosome Location : 12p13
Pathway : Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem;
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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