Recombinant Human CD27 protein, His-tagged

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Recombinant Human CD27 protein, His-tagged

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Cat.No. : CD27-6271H
Product Overview : Recombinant Human CD27 (Gln39-Pro193) protein was fused to His-tag at N-terminus and expressed in human 293 cells (HEK293).
Description : Cluster of Differentiation 70 (CD70) is also known as CD27 ligand (CD27L/CD27LG), TNFSF7, TNFSF7G, is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and is a surface antigen found on activated T-and B-lymphocytes and mature dendritic cells. Binding of CD70 to its receptor CD27 induces in priming, effector functions, differentiation and memory formation of T-cells, and thus is invloved in the biological processes including T-cell activation, the proliferation of costimulated T-cells, as well as the generation of cytolytic T-cells. CD70 on T cells provides costimulatory signals that are required for T cell proliferation, clonal expansion and the promotion of effector T cell formation. CD70 on mouse B cell has been shown to inhibit terminal differentiation of activated B cells into plasma cells and enhances commitment to memory B cell responses. CD70 induces proliferation and IFNγ production, on NK cells.
Source : HEK293
Species : Human
Tag : His
Predicted N Terminal : Gln39
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 19.0 kDa. The protein migrates as 30-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Gln39-Pro193
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >90% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of CD27-6271H :

Human CD27 Ligand, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA of CD27-6271H :

Immobilized Human CD27, Fc Tag at 2 μg/mL (100 μL/well) can bind Human CD27 Ligand, His Tag with a linear range of 0.013-0.1 μg/mL (QC tested).

Bioactivity-ELISA :

Batch consistency of Bioactivity-ELISA

Gene Name : CD27
Official Symbol : CD27
Synonyms : CD27; CD27 molecule; TNFRSF7, tumor necrosis factor receptor superfamily, member 7; CD27 antigen; S152; Tp55; CD27L receptor; T cell activation antigen S152; T-cell activation antigen CD27; tumor necrosis factor receptor superfamily, member 7; T14; TNFRSF7; MGC20393
Gene ID : 939
mRNA Refseq : NM_001242
Protein Refseq : NP_001233
MIM : 186711
UniProt ID : P26842

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