Active Recombinant Human CD276 protein, His-tagged

• Cat.No.: CD276-3884H
• Product Overview: Active Recombinant Human CD276 protein(Q5ZPR3-1)(Gly27-Thr461) is expressed from HEK293 with His tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 27-461 aa
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Human B7-H3 (4Ig), His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-B7-H3 Antibody, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 48.25 kDa. Due to glycosylation, the protein migrates to 68-78 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.
• protein Refseq-Weblink: http://www.ncbi.nlm.nih.gov/protein/NP_001019907.1
• Unit ID: Q5ZPR3
• Unit ID-Weblink: https://www.uniprot.org/uniprotkb/Q5ZPR3

Gene Information

• Gene Name: CD276 CD276 molecule [ Homo sapiens ]
• Official Symbol: CD276
• Synonyms: CD276; CD276 molecule; CD276 antigen; B7 H3; B7H3; B7RP 2; B7 homolog 3; costimulatory molecule; B7-H3; B7RP-2; 4Ig-B7-H3
• Gene ID: 80381
• mRNA Refseq: NM_001024736
• protein Refseq: NP_001019907
• MIM: 605715