Active Recombinant Human CD300A protein, hFc-tagged, Low Endotoxin

• Cat.No.: CD300A-7854H
• Product Overview: Recombinant Human CD300A protein(Q9UGN4-1)(Leu18-Pro180), fused with C-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Protein Length: 18-180 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human CD300A, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD300A Antibody, hFc Tag with the EC50 of 57.4ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 44.4 kDa. Due to glycosylation, the protein migrates to 60-75 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: CD300A CD300a molecule [ Homo sapiens ]
• Official Symbol: CD300A
• Synonyms: CD300A; CD300a molecule; CD300a antigen; CMRF35-like molecule 8; CMRF 35 H9; CMRF35H; IGSF12; IRC1; IRC2; Irp60; NK inhibitory receptor; leukocyte membrane antigen; inhibitory receptor protein 60; CD300 antigen-like family member A; immunoglobulin superfamily member 12; CMRF35H leukocyte immunoglobulin-like receptor; CLM-8; IRp60; CMRF-35H; CMRF35-H; CMRF35H9; CMRF35-H9; IRC1/IRC2; CMRF-35-H9
• Gene ID: 11314
• mRNA Refseq: NM_001256841
• Protein Refseq: NP_001243770
• MIM: 606790
• UniProt ID: Q9UGN4