Active Recombinant Human CD33 protein, Avi-His-tagged, Biotinylated

• Cat.No.: CD33-268H
• Product Overview: Biotinylated Recombinant Human CD33 protein(Asp 18 - His 259), fused with Avi and His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: Avi-His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Bio-activity: Immobilized anti human CD33 MAb, Mouse IgG1 (Clone # 6C5/2) at 2 μg/mL (100 μL/well) can bind Biotinylated Human Siglec-3, Avitag,His Tag with a linear range of 0.078-2.5 ng/mL.
• Molecular Mass: This protein carries an Avi tag (Avitag) at the C-terminus, followed by a polyhistidine tag.The protein has a calculated MW of 29.4 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein Length: Asp 18 - His 259
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CD33 CD33 molecule [ Homo sapiens ]
• Official Symbol: CD33
• Synonyms: CD33; CD33 molecule; CD33 antigen (gp67); myeloid cell surface antigen CD33; FLJ00391; p67; sialic acid binding Ig like lectin 3; SIGLEC 3; SIGLEC3; gp67; sialic acid binding Ig-like lectin 3; sialic acid-binding Ig-like lectin 3; SIGLEC-3
• Gene ID: 945
• mRNA Refseq: NM_001082618
• Protein Refseq: NP_001076087
• MIM: 159590
• UniProt ID: P20138