Recombinant Human CD3E, His tagged

• Cat.No.: CD3E-2194H
• Product Overview: Recombinant Human CD3E (NP_000724.1) precursor extracellular domain (Met 1-Asp 126), fused with a polyhistidine tag at the C-terminus, was produced in Human Cell.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Met 1-Asp 126
• Form: Lyophilized from sterile PBS, pH 7.4
• Molecular Mass: The recombinant human CD3E consists of 115 amino acids after removal of the signal peptide and has a predicted molecular mass of 13.2 kDa. As a result of glycosylation, human CD3E migrates as an approximately 22.3 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 80 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CD3E CD3e molecule, epsilon (CD3-TCR complex) [ Homo sapiens ]
• Official Symbol: CD3E
• Synonyms: CD3E; CD3e molecule, epsilon (CD3-TCR complex); CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell antigen receptor complex, epsilon subunit of T3; T3E; TCRE; FLJ18683
• Gene ID: 916
• mRNA Refseq: NM_000733
• Protein Refseq: NP_000724
• UniProt ID: P07766