Recombinant Human CD3E protein, His-tagged

• Cat.No.: CD3E-213H
• Product Overview: Recombinant Human CD3E protein(P07766)(Asp23-Asp126), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Asp23-Asp126
• Tag: C-His
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human CD3E, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 9.0 ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 12.6 kDa. Due to glycosylation, the protein migrates to 15 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
• Publications:
  Efficient tumor killing and minimal cytokine release with novel T-cell agonist bispecific antibodies (2019)
  Targeting Dual Immune Checkpoints PD-L1 and HLA-G by Trispecific T Cell Engager for Treating Heterogeneous Lung Cancer (2024)

Gene Information

• Gene Name: CD3E CD3e molecule, epsilon (CD3-TCR complex) [ Homo sapiens ]
• Official Symbol: CD3E
• Synonyms: CD3E; CD3e molecule, epsilon (CD3-TCR complex); CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell antigen receptor complex, epsilon subunit of T3; T3E; TCRE; FLJ18683
• Gene ID: 916
• mRNA Refseq: NM_000733
• Protein Refseq: NP_000724
• UniProt ID: P07766

Citation

Efficient tumor killing and minimal cytokine release with novel T-cell agonist bispecific antibodies

Journal: mAbs    PubMed ID: 30698484    Data: 2019/6/1

Authors: Nathan D. Trinklein, Duy Pham, Shelley Force Aldred

Article Snippet:C-terminal polyhistidine (His) tags on CD3δ and CD3γ were used for binding and imidazole-gradient elution of heterodimers from Ni-Sepharose XL (GE Healthcare).C-terminal polyhistidine (His) tags on CD3δ and CD3γ were used for binding and imidazole-gradient elution of heterodimers from Ni-Sepharose XL (GE Healthcare).. His-tagged human CD3? was purchased from Creative BioMart (#CD3E-213H).. Antigen-antibody binding kinetics analysis was performed on the Octet QK-384 (ForteBio).Antigen-antibody binding kinetics analysis was performed on the Octet QK-384 (ForteBio).