Recombinant Human CD3E protein, His-tagged

• Cat.No.: CD3E-213H
• Product Overview: Recombinant Human CD3E protein(P07766)(Asp23-Asp126), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His
• Protein length: Asp23-Asp126
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human CD3E, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 9.0 ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 12.6 kDa. Due to glycosylation, the protein migrates to 15 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: CD3E CD3e molecule, epsilon (CD3-TCR complex) [ Homo sapiens ]
• Official Symbol: CD3E
• Synonyms: CD3E; CD3e molecule, epsilon (CD3-TCR complex); CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell antigen receptor complex, epsilon subunit of T3; T3E; TCRE; FLJ18683
• Gene ID: 916
• mRNA Refseq: NM_000733
• Protein Refseq: NP_000724
• UniProt ID: P07766

Citation

Efficient tumor killing and minimal cytokine release with novel T-cell agonist bispecific antibodies

Journal: mAbs    PubMed ID: 30698484    Data: 2019/6/1

Authors: Nathan D. Trinklein, Duy Pham, Shelley Force Aldred

Article Snippet:C-terminal polyhistidine (His) tags on CD3δ and CD3γ were used for binding and imidazole-gradient elution of heterodimers from Ni-Sepharose XL (GE Healthcare).C-terminal polyhistidine (His) tags on CD3δ and CD3γ were used for binding and imidazole-gradient elution of heterodimers from Ni-Sepharose XL (GE Healthcare).. His-tagged human CD3? was purchased from Creative BioMart (#CD3E-213H).. Antigen-antibody binding kinetics analysis was performed on the Octet QK-384 (ForteBio).Antigen-antibody binding kinetics analysis was performed on the Octet QK-384 (ForteBio).

Publication :

Efficient tumor killing and minimal cytokine release with novel T-cell agonist bispecific antibodies (2019)

Targeting Dual Immune Checkpoints PD-L1 and HLA-G by Trispecific T Cell Engager for Treating Heterogeneous Lung Cancer (2024)

Reviews

03/22/2023

Manufacturers with the capacity for bulk production can offer CD3E protein in larger quantities, which is advantageous for researchers working on large-scale experiments or collaborations.

09/12/2021

bulk production often leads to economies of scale, making the CD3E protein more cost-effective for research budgets.

06/17/2018

Manufacturers can actively seek collaborations and partnerships with researchers to develop new applications or optimize methodologies using CD3E protein.

Q&As

Can CD3E be used to assess the effectiveness of cancer immunotherapies?

Yes, CD3E levels and T-cell function can be monitored to evaluate the response to cancer immunotherapies like checkpoint inhibitors.

Are there any CD3E-targeted therapies?

There are currently no approved therapies that directly target CD3E, but it is a target for research in developing immunotherapies and cancer treatments.

Are there any ongoing clinical trials involving CD3E?

There may be ongoing clinical trials using CD3E-targeted therapies in the context of cancer and autoimmune diseases. It's important to check clinical trial databases for specific studies.

Can CD3E levels be used to monitor the progression of HIV?

Yes, monitoring CD3E levels in HIV-infected individuals can provide valuable information about the progression of the disease and the effectiveness of antiretroviral therapy.

How is CD3E used in allergy diagnostics?

CD3E can be used in research to study T-cell responses in allergic conditions, helping to better understand and diagnose allergies.