| Description : |
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region. |
| Source : |
HEK293 |
| Species : |
Monkey |
| Tag : |
C-Fc |
| Form : |
Lyophilized, Sterile-filtered colorless solution |
| Molecular Mass : |
22149 kDa |
| Protein Length : |
198 aa |
| AA Sequence : |
MQSGTRWRVLGLCLLSIGVWGQDGN EEMGSITQTPYQVSISGTTVILTCS QHLGSEAQWQHNGKNKEDSGDRLFL PEFSEMEQSGYYVCYPRGSNPEDAS HHLYLKARVCENCMEMDVMAVATIV IVDICITLGLLLLVYYWSKNRKAKA KPVTRGAGAGGRQRGQNKERPPPVP NPDYEPIRKGQQDLYSGLNQRRI |
| Purity : |
>98% |
| Stability : |
This bioreagent is stable at 4 centigrade (short-term) and -70 centigrade(long-term). After reconstitution, sample may be stored at 4 centigrade for 2-7 days and below -18 centigrade for future use. |
| Storage : |
At -20 centigrade |
| Concentration : |
1 mg/mL |
| Storage Buffer : |
PBS, pH 7.4-7.5 |
| Reconstitution : |
Reconstitute in sterile distilled H2O to no less than 100 μg/mL; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance. |
