Recombinant Human CD3E Protein, C-His-tagged

• Cat.No.: CD3E-169H
• Product Overview: Recombinant Human CD3E Protein with C-His tag was expressed in E. coli.

Specification

• Description: When T cells encounter antigens via the T cell receptor (TCR), information about the quantity and quality of antigens is relayed to the intracellular signal transduction machinery. This activation process depends mainly on CD3 (Cluster of Differentiation 3), a multiunit protein complex that directly associates with the TCR. CD3 is composed of four polypeptides: ζ, γ, ε and δ. Each of these polypeptides contains at least one immunoreceptor tyrosine-based activation motif (ITAM). Engagement of TCR complex with foreign antigens induces tyrosine phosphorylation in the ITAM motifs and phosphorylated ITAMs function as docking sites for signaling molecules such as ZAP-70 and p85 subunit of PI-3 kinase. TCR ligation also induces a conformational change in CD3ε, such that a proline region is exposed and then associates with the adaptor protein Nck.
• Source: E. coli
• Species: Human
• Tag: His
• Molecular Mass: ~11 kDa
• AA Sequence: DGNEEMGGITQTPYKVSISGTTVIL TCPQYPGSEILWQHNDKNIGGDEDD KNIGSDEDHLSLKEFSELEQSGYYV CYPRGSKPEDANFYLYLRARVCENC MEMD
• Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).
• Notes: For research use only, not for use in diagnostic procedure.
• Storage: Store at 4 centigrade short term. Aliquot and store at -20 centigrade long term. Avoid freeze-thaw cycles.
• Concentration: ≥0.5 mg/mL
• Storage Buffer: PBS, 4M Urea, pH7.4

Gene Information

• Gene Name: CD3E CD3e molecule, epsilon (CD3-TCR complex) [ Homo sapiens (human) ]
• Official Symbol: CD3E
• Synonyms: CD3E; CD3e molecule, epsilon (CD3-TCR complex); CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell antigen receptor complex, epsilon subunit of T3; T3E; TCRE; FLJ18683
• Gene ID: 916
• mRNA Refseq: NM_000733
• Protein Refseq: NP_000724
• MIM: 186830
• UniProt ID: P07766

Reviews

03/22/2023

Manufacturers with the capacity for bulk production can offer CD3E protein in larger quantities, which is advantageous for researchers working on large-scale experiments or collaborations.

09/12/2021

bulk production often leads to economies of scale, making the CD3E protein more cost-effective for research budgets.

06/17/2018

Manufacturers can actively seek collaborations and partnerships with researchers to develop new applications or optimize methodologies using CD3E protein.

Q&As

Can CD3E be used to assess the effectiveness of cancer immunotherapies?

Yes, CD3E levels and T-cell function can be monitored to evaluate the response to cancer immunotherapies like checkpoint inhibitors.

Are there any CD3E-targeted therapies?

There are currently no approved therapies that directly target CD3E, but it is a target for research in developing immunotherapies and cancer treatments.

Are there any ongoing clinical trials involving CD3E?

There may be ongoing clinical trials using CD3E-targeted therapies in the context of cancer and autoimmune diseases. It's important to check clinical trial databases for specific studies.

Can CD3E levels be used to monitor the progression of HIV?

Yes, monitoring CD3E levels in HIV-infected individuals can provide valuable information about the progression of the disease and the effectiveness of antiretroviral therapy.

How is CD3E used in allergy diagnostics?

CD3E can be used in research to study T-cell responses in allergic conditions, helping to better understand and diagnose allergies.