|Product Overview :||Biotinylated Human CD4, His Tag is expressed from human 293 cells (HEK293). It contains AA Lys 26 - Pro 396. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The single lysine residue in the Avitag is enzymatically labeled with biotin.|
|Description :||T-cell surface glycoprotein CD4 is also known as T-cell surface antigen T4/Leu-3. CD4 contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CD4 is accessory protein for MHC class-II antigen/T-cell receptor interaction. CD4 induces the aggregation of lipid rafts. CD4 is a primary receptor used by HIV-1 to gain entry into host T cells. HIV infection leads to a progressive reduction of the number of T cells possessing CD4 receptors. Therefore, medical professionals refer to the CD4 count to decide when to begin treatment for HIV-infected patients.|
|Tag :||His & Avi|
|Predicted N Terminal :||Lys 26|
|Molecular Mass :||The protein has a calculated MW of 45.0 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.|
|Endotoxin :||Less than 1.0 EU per μg by the LAL method.|
|Purity :||>95% as determined by SDS-PAGE.|
|Storage :||For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Storage Buffer :||Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.|
Biotinylated Human CD4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
|Gene Name :||CD4|
|Official Symbol :||CD4 CD4 molecule [ Homo sapiens (human) ]|
|Synonyms :||CD4; CD4mut; LEU3; CD4 molecule; T-cell surface glycoprotein CD4; CD4 antigen (p55); CD4 receptor; T-cell surface antigen T4/Leu-3|
|Gene ID :||920|
|mRNA Refseq :||NM_000616|
|Protein Refseq :||NP_000607|
|UniProt ID :||P01730|