Recombinant Human CD4 Protein, His & Avi-tagged

• Cat.No.: CD4-24H
• Product Overview: Biotinylated Human CD4, His Tag is expressed from human 293 cells (HEK293). It contains AA Lys 26 - Pro 396. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The single lysine residue in the Avitag is enzymatically labeled with biotin.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: 26-396 a.a.
• Description: T-cell surface glycoprotein CD4 is also known as T-cell surface antigen T4/Leu-3. CD4 contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CD4 is accessory protein for MHC class-II antigen/T-cell receptor interaction. CD4 induces the aggregation of lipid rafts. CD4 is a primary receptor used by HIV-1 to gain entry into host T cells. HIV infection leads to a progressive reduction of the number of T cells possessing CD4 receptors. Therefore, medical professionals refer to the CD4 count to decide when to begin treatment for HIV-infected patients.
• Predicted N Terminal: Lys 26
• Molecular Mass: The protein has a calculated MW of 45.0 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Gene Information

• Gene Name: CD4
• Official Symbol: CD4 CD4 molecule [ Homo sapiens (human) ]
• Synonyms: CD4; CD4mut; LEU3; CD4 molecule; T-cell surface glycoprotein CD4; CD4 antigen (p55); CD4 receptor; T-cell surface antigen T4/Leu-3
• Gene ID: 920
• mRNA Refseq: NM_000616
• Protein Refseq: NP_000607
• MIM: 186940
• UniProt ID: P01730