Active Recombinant Human CD5 protein, His-tagged

• Cat.No.: CD5-2222H
• Product Overview: Active Recombinant Human CD5 protein(NP_055022.2)(Met 1- 372) was expressed in HEK293 with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-372 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its ability to bind biotinylated recombinant human LCK in a functional ELISA.
• Molecular Mass: The secreted recombinant human CD5 consists of 359 amino acids after removal of the signal peptide and has a calculated molecular mass of 40 kDa. In SDS-PAGE under reducing conditions, it migrates as an apximately 47 kDa band due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• protein Refseq-Weblink: http://www.ncbi.nlm.nih.gov/protein/NP_055022.2
• Unit ID: P06127
• Unit ID-Weblink: http://www.uniprot.org/uniprot/P06127

Gene Information

• Gene Name: CD5 CD5 molecule [ Homo sapiens ]
• Official Symbol: CD5
• Synonyms: CD5; CD5 molecule; CD5 antigen (p56 62) , LEU1; T-cell surface glycoprotein CD5; T1; CD5 antigen (p56-62); lymphocyte antigen T1/Leu-1; LEU1
• Gene ID: 921
• mRNA Refseq: NM_014207
• protein Refseq: NP_055022
• MIM: 153340