Recombinant Human CD52 Protein, Fc-Avi-tagged, Biotinylated CD52-390H

Recombinant Human CD52 Protein, Fc-Avi-tagged, Biotinylated

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Cat.No. : CD52-390H Optional Service: Optional requirements on this protein
Product Overview : Biotinylated Human CD52, Fc Tag is expressed from HEK293 cells. It contains AA Gly 25 - Ser 36 (Accession # P31358-1 ). This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag.
Source : HEK293
Species : Human
Tag : Fc-Avi
Predicted N Terminal : Gly 25
Form : Lyophilized from 0.22 um filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
Bio-activity : Immobilized Anti CD52 MAb, Human IgG1 at 10 ug/mL (100 uL/well) can bind Biotinylated Human CD52, Fc Tag with a linear range of 0.625-10 ug/mL.
Molecular Mass : The protein has a calculated MW of 29.7 kDa. As a result of glycosylation, the protein migrates as 38-45 kDa under reducing condition, and 66-100 kDa under non-reducing condition (SDS-PAGE).
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Characteristic : Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
Stability : Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage : For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name : CD52 CD52 molecule [ Homo sapiens ]
Official Symbol : CD52
Synonyms : CD52; CD52 molecule; CD52 antigen (CAMPATH 1 antigen) , CDW52; CAMPATH-1 antigen; he5; cambridge pathology 1 antigen; epididymal secretory protein E5; CD52 antigen (CAMPATH-1 antigen); CDW52 antigen (CAMPATH-1 antigen); human epididymis-specific protein 5; CDW52;
Gene ID : 1043
mRNA Refseq : NM_001803
Protein Refseq : NP_001794
MIM : 114280
UniProt ID : P31358
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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