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Recombinant Human CD52 Protein, Fc-Avi-tagged, Biotinylated

Cat.No. : CD52-390H
Product Overview : Biotinylated Human CD52, Fc Tag is expressed from HEK293 cells. It contains AA Gly 25 - Ser 36 (Accession # P31358-1 ). This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag.
Source : HEK293
Species : Human
Tag : Fc-Avi
Predicted N Terminal : Gly 25
Form : Lyophilized from 0.22 um filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
Bio-activity : Immobilized Anti CD52 MAb, Human IgG1 at 10 ug/mL (100 uL/well) can bind Biotinylated Human CD52, Fc Tag with a linear range of 0.625-10 ug/mL.
Molecular Mass : The protein has a calculated MW of 29.7 kDa. As a result of glycosylation, the protein migrates as 38-45 kDa under reducing condition, and 66-100 kDa under non-reducing condition (SDS-PAGE).
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Characteristic : Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
Stability : Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage : For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name : CD52 CD52 molecule [ Homo sapiens ]
Official Symbol : CD52
Synonyms : CD52; CD52 molecule; CD52 antigen (CAMPATH 1 antigen) , CDW52; CAMPATH-1 antigen; he5; cambridge pathology 1 antigen; epididymal secretory protein E5; CD52 antigen (CAMPATH-1 antigen); CDW52 antigen (CAMPATH-1 antigen); human epididymis-specific protein 5; CDW52;
Gene ID : 1043
mRNA Refseq : NM_001803
Protein Refseq : NP_001794
MIM : 114280
UniProt ID : P31358

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