Recombinant Human CD55, His-tagged

• Cat.No.: CD55-26634TH
• Product Overview: Recombinant fragment: LPPDVPNAQP ALEGRTSFPE DTVITYKCEE SFVKIPGEKD SVICLKGSQW SDIEEFCNR, corresponding to amino acids 38-96 of Human CD55 fused to a His tag at N-terminal end, 11kDa.

Specification

• Description: This gene encodes a protein involved in the regulation of the complement cascade. The encoded glycoprotein is also known as the decay-accelerating factor (DAF); binding of DAF to complement proteins accelerates their decay, disrupting the cascade and preventing damage to host cells. Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM). Two alternatively spliced transcripts encoding different proteins have been identified. The predominant transcript encodes a membrane-bound protein expressed on cells exposed to plasma component proteins but an alternatively spliced transcript produces a soluble protein present at much lower levels. Additional, alternatively spliced transcript variants have been described, but their biological validity has not been determined.
• Conjugation: HIS
• Source: E. coli
• Tissue specificity: Expressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, and variants of the molecule are present in body flui
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: PBS
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Sequences of amino acids: LPPDVPNAQPALEGRTSFPEDTVIT YKCEESFVKIPGEKDSVICLKGSQW SDIEEFCNR
• Sequence Similarities: Belongs to the receptors of complement activation (RCA) family.Contains 4 Sushi (CCP/SCR) domains.

Gene Information

• Gene Name: CD55 CD55 molecule, decay accelerating factor for complement (Cromer blood group) [ Homo sapiens ]
• Official Symbol: CD55
• Synonyms: CD55; CD55 molecule, decay accelerating factor for complement (Cromer blood group); DAF, decay accelerating factor for complement (CD55, Cromer blood group system); complement decay-accelerating factor; CR; CROM; TC
• Gene ID: 1604
• mRNA Refseq: NM_000574
• Protein Refseq: NP_000565
• MIM: 125240
• Uniprot ID: P08174
• Chromosome Location: 1q32
• Pathway: Class B/2 (Secretin family receptors), organism-specific biosystem; Complement Activation, Classical Pathway, organism-specific biosystem; Complement and coagulation cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; GPCR ligand binding, organism-specific biosystem
• Function: receptor activity

Reviews

02/23/2022

Efficient shipping and exceptional product quality. This company knows how to serve its customers.

09/20/2019

Consistency is key in research, and this product delivers that every time.

03/02/2018

We've tried various proteins, but this one stands out for its purity and performance.

Q&As

How do genetic variations in CD55 influence susceptibility to complement-mediated disorders, and what are the molecular mechanisms involved?

Genetic variations in CD55 may influence susceptibility to complement-mediated disorders by affecting the protein's ability to regulate complement activation. This can lead to conditions like paroxysmal nocturnal hemoglobinuria (PNH).

How does CD55 contribute to the modulation of immune responses beyond its role in complement regulation?

CD55 has immunomodulatory functions beyond complement regulation, influencing T cell responses and contributing to the maintenance of immune tolerance.

What role does CD55 play in protecting cells from complement-mediated lysis, and how does it contribute to immune evasion?

CD55 protects cells from complement-mediated lysis by preventing the formation of membrane attack complexes. This contributes to immune evasion by pathogens and self-cells alike.

Can you elaborate on the structural domains of CD55 and how they mediate its interactions with complement components?

CD55 consists of extracellular domains responsible for interactions with complement proteins, particularly C3b and C4b. These interactions prevent the formation of membrane attack complexes on host cells.

Can you discuss the involvement of CD55 in various diseases, including autoimmune disorders and infectious diseases?

CD55 dysregulation is associated with autoimmune disorders, where it may contribute to immune system overactivity. Additionally, some pathogens exploit CD55 to evade host immune responses.