Recombinant Human CD55 Protein, His-tagged

• Cat.No.: CD55-164H
• Product Overview: Recombinant human CD55 protein with His tag was expressed in HEK293.

Specification

• Description: This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM). Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins.
• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized
• Molecular Mass: 35.8 kDa
• Protein length: 381
• AA Sequence: MTVARPSVPAALPLLGELPRLLLLV LLCLPAVWGDCGLPPDVPNAQPALE GRTSFPEDTVITYKCEESFVKIPGE KDSVICLKGSQWSDIEEFCNRSCEV PTRLNSASLKQPYITQNYFPVGTVV EYECRPGYRREPSLSPKLTCLQNLK WSTAVEFCKKKSCPNPGEIRNGQID VPGGILFGATISFSCNTGYKLFGST SSFCLISGSSVQWSDPLPECREIYC PAPPQIDNGIIQGERDHYGYRQSVT YACNKGFTMIGEHSIYCTVNNDEGE WSGPPPECRGKSLTSKVPPTVQKPT TVNVPTTEVSPTSQKTTTKTTTPNA QATRSTPVSRTTKHFHETTPNKGSG TTSGTTRLLSGHTCFTLTGLLGTLV TMGLLT
• Purity: > 98%
• Applications: WB; ELISA; FACS; FC
• Stability: This bioreagent is stable at 4 centigrade (short-term) and -70 centigrade(long-term). After reconstitution, sample may be stored at 4 centigrade for 2-7 days and below -18 centigrade for future use.
• Storage: At -20 centigrade.
• Concentration: 1 mg/mL
• Storage Buffer: PBS (pH 7.4-7.5). Sterile-filtered colorless solution.
• Reconstitution: Reconstitute in sterile distilled H2O to no less than 100 μg/mL; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.

Gene Information

• Gene Name: CD55 CD55 molecule, decay accelerating factor for complement (Cromer blood group) [ Homo sapiens (human) ]
• Official Symbol: CD55
• Synonyms: CD55; CD55 molecule, decay accelerating factor for complement (Cromer blood group); DAF, decay accelerating factor for complement (CD55, Cromer blood group system); complement decay-accelerating factor; CR; CROM; TC; CD55 antigen; DAF
• Gene ID: 1604
• mRNA Refseq: NM_000574
• Protein Refseq: NP_000565
• MIM: 125240
• UniProt ID: P08174

Reviews

02/23/2022

Efficient shipping and exceptional product quality. This company knows how to serve its customers.

09/20/2019

Consistency is key in research, and this product delivers that every time.

03/02/2018

We've tried various proteins, but this one stands out for its purity and performance.

Q&As

How do genetic variations in CD55 influence susceptibility to complement-mediated disorders, and what are the molecular mechanisms involved?

Genetic variations in CD55 may influence susceptibility to complement-mediated disorders by affecting the protein's ability to regulate complement activation. This can lead to conditions like paroxysmal nocturnal hemoglobinuria (PNH).

How does CD55 contribute to the modulation of immune responses beyond its role in complement regulation?

CD55 has immunomodulatory functions beyond complement regulation, influencing T cell responses and contributing to the maintenance of immune tolerance.

What role does CD55 play in protecting cells from complement-mediated lysis, and how does it contribute to immune evasion?

CD55 protects cells from complement-mediated lysis by preventing the formation of membrane attack complexes. This contributes to immune evasion by pathogens and self-cells alike.

Can you elaborate on the structural domains of CD55 and how they mediate its interactions with complement components?

CD55 consists of extracellular domains responsible for interactions with complement proteins, particularly C3b and C4b. These interactions prevent the formation of membrane attack complexes on host cells.

Can you discuss the involvement of CD55 in various diseases, including autoimmune disorders and infectious diseases?

CD55 dysregulation is associated with autoimmune disorders, where it may contribute to immune system overactivity. Additionally, some pathogens exploit CD55 to evade host immune responses.