Active Recombinant Human CD74 protein, His-tagged, Low Endotoxin

• Cat.No.: CD74-2227H
• Product Overview: Recombinant Human CD74 protein(NP_004346.1)(Gln73-Met232), fused with N-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 73-232 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human CD74 at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD74 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 19.1 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 90% as determined by HPLC
• Storage: Store at -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: CD74 CD74 molecule, major histocompatibility complex, class II invariant chain [ Homo sapiens ]
• Official Symbol: CD74
• Synonyms: CD74; CD74 molecule, major histocompatibility complex, class II invariant chain; CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen associated), DHLAG; HLA class II histocompatibility antigen gamma chain; gamma chain of class II antigens; HLA DR gamma; Ia associated invariant chain; MHC HLA DR gamma chain; p33; HLA-DR-gamma; MHC HLA-DR gamma chain; Ia-associated invariant chain; HLA-DR antigens-associated invariant chain; CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated); II; DHLAG; HLADG; Ia-GAMMA; FLJ98970
• Gene ID: 972
• mRNA Refseq: NM_001025158
• Protein Refseq: NP_001020329
• MIM: 142790
• UniProt ID: P04233