Recombinant Human CD82 cell lysate

• Cat.No.: CD82-1960HCL
• Product Overview: Human CD82 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Human cells
• Species: Human
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
• Tag: Non

Gene Information

• Gene Name: CD82 CD82 antigen (R2 leukocyte antigen, antigen detected by monoclonal [ Homo sapiens ]
• Official Symbol: CD82
• Synonyms: CD82; CD82 antigen (R2 leukocyte antigen, antigen detected; CD82 antigen (R2 leukocyte antigen, antigen detected by monoclonal; SAR2
• Gene ID: 8052

Reviews

02/28/2021

Integral to our research. Highly recommended service.

10/22/2020

Exceptional accuracy and support. A research necessity.

08/26/2018

Boosts research efficiency. Contributes to discoveries.

Q&As

How do genetic variations in CD82 affect cancer progression?

Genetic mutations in CD82 can lead to reduced tumor-suppressive functions, enhancing the risk of cancer spread.

What potential therapeutic applications arise from targeting CD82 in cancer treatment?

Targeting CD82 in cancer therapy could exploit its tumor-suppressive properties to prevent metastasis.

How does CD82 interact with other membrane proteins in signaling pathways?

CD82 collaborates with other membrane proteins to regulate various signaling pathways, impacting cell behavior.

What is the primary role of CD82 in cell signaling and communication?

CD82, a member of the tetraspanin family, is involved in modulating cell signaling and intercellular communication.

What is the impact of altered CD82 activity on immune response?

Altered CD82 activity can influence immune cell interactions and responses.

What role does CD82 play in tumor suppression and metastasis prevention?

CD82 acts as a tumor suppressor, inhibiting cancer cell migration and metastasis.

How does CD82 contribute to the regulation of cell migration and adhesion?

It plays a crucial role in regulating cell migration and adhesion, key processes in tissue organization and wound healing.