|Product Overview :||Recombinant Human CD96 protein (Val22-Met503)(low endotoxin), fused to Mouse IgG2a Fc tag at C-terminus, was expressed in human 293 cells (HEK293).|
|Description :||The progression of pancreatic cancer (PC) is significantly associated with tumor immune escape, which may be associated with nature killer (NK) cell dysfunction. CD226, CD96, and TIGIT, which share the ligand CD155, play important roles in the regulation of NK cell function. The present study was conducted to investigate the roles of these molecules in NK cells from PC patients.
TIGIT and CD96 together with the co-stimulatory receptor CD226 form a pathway that is analogous to the CD28/CTLA-4 pathway, in which shared ligands and differential receptor:ligand affinities fine-tune the immune response. Although the roles of TIGIT and CD96 as immune checkpoint receptors in T cell and natural killer cell biology are just beginning to be uncovered, accumulating data support the targeting of these receptors for improving anti-tumor immune responses. A clear understanding of the immune cell populations regulated by TIGIT and CD96 is key to the design of immunotherapies that target these receptors in combination with other existing immune checkpoint blockade therapies.
The dysfunction of CD96 may trigger C syndrome: A syndrome characterized by trigonocephaly, severe mental retardation, hypotonia, variable cardiac defects, redundant skin, and dysmorphic facial features, including upslanted palpebral fissures, epicanthal folds, depressed nasal bridge, and low-set, posteriorly rotated ears.
|Tag :||Mouse IgG2a Fc|
|Form :||Lyophilized from 0.22 um filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.|
|Molecular Mass :||The protein has a calculated MW of 80.4 kDa. The protein migrates as 110-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.|
|Protein length :||482|
|Endotoxin :||Less than 0.1 EU per ug by the LAL method.|
|Purity :||>90% as determined by reduced SDS-PAGE.|
|Storage :||For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 centigrade to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
|SDS-PAGE of CD96-357H :||
Human CD96, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
|Bioactivity-ELISA of CD96-357H :||
Immobilized Human CD96, Mouse IgG2a Fc Tag, low endotoxin at 2 μg/mL (100 μL/well) can bind Human CD155, Fc Tag with a linear range of 0.078-1.25 μg/mL (QC tested).
|Gene Name :||CD96|
|Official Symbol :||CD96|
|Synonyms :||CD96; CD96 molecule; CD96 antigen; T-cell surface protein tactile; TACTILE; cell surface antigen CD96; T cell activation, increased late expression; t cell-activated increased late expression protein; MGC22596; DKFZp667E2122|
|Gene ID :||10225|
|mRNA Refseq :||NM_005816|
|Protein Refseq :||NP_005807|
|UniProt ID :||P40200|